The role of colloids in the migration of radionuclides in the geosphere has been emphasized in the performance assessment of high-level radioactive waste disposal. The literature indicates that the colloid velocity may not be equal to the velocity of groundwater owing to hydrodynamic chromatography. A theoretical model for hydrodynamic chromatography of colloid migration in the fracture is proposed in the present work. In this model, the colloids are treated as nonreactive and the external forces acting on colloidal particles are considered including the inertial force, the van der Waals attractive force, and the electrical double-layer repulsive force, as well as the gravitational force. A fully developed concentration profile for colloids is obtained to elucidate migration behavior for colloids in the fracture. The effects of parameters governing these forces and the aperture of the fracture are determined using a theoretical model.