MARS is a best-estimate system analysis code that is based on the RELAP5/MOD3 and COBRA-TF codes. The COBRA-TF code was adapted as a three-dimensional thermal-hydraulic module in MARS. It uses a two-fluid, three-field model for two-phase flows and has a subchannel flow mixing model. The subchannel flow mixing model of the MARS three-dimensional module was assessed by using the ISPRA 16-rod bundle test and the GE 9-rod bundle test data. These tests represent typical pressurized water reactor and boiling water reactor core thermal-hydraulic conditions, respectively. Two interconnected subchannel tests that were performed under atmospheric pressure conditions were also used for the assessment. From the results of the assessments, a simple modification of the subchannel flow mixing model was suggested to take into account the effects of the system pressure on the void drift phenomena.