The aim of this study was to validate a Monte Carlo-based model of the Greek Research Reactor-1 (GRR-1) developed with the MCNP5 code. The GRR-1 core was modeled in detail using the exact geometry without approximations. The inventory of the core was derived using the WIMS-ANL code, taking into account the different 235U burnup of each fuel assembly. The model was validated against experimentally determined control rod reactivity worth and neutron flux measurements performed in various irradiation positions. The ratio of the calculated-to-measured integral reactivity of each of the five control rods was found to be 0.972 ± 0.151, 1.083 ± 0.168, 1.156 ± 0.179, 0.874 ± 0.137, and 1.097 ± 0.170. The calculated-to-measured thermal neutron flux ratios ranged from 0.83 ± 0.04 to 1.22 ± 0.07. Therefore, good agreement between MCNP calculated and experimental values was observed. The GRR-1 core model will be fully implemented in the design of material irradiation experiments along with reactor safety and fuel management studies.