A computer-based Expert Adviser has been developed to catalog technical expertise and dispense this knowledge to operators of a complex system of distillation columns at the Los Alamos National Laboratory (LANL). The Expert Adviser is also used to train personnel in operation of the LANL Tritium Systems Test Assembly (TSTA) Isotope Separations System (ISS). The methodology is applicable to a wide range of operations where documentation and transfer of knowledge is desirable.