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Scavenging Activity of Tea Catechins on Hydroxyl Radical Formed in Tritiated Water Studied by the Solid-State Spin Trapping Method

Mariko Konishi, Yasunori Ohashi, Hiroe Yoshioka, Hisashi Yoshioka

Fusion Science and Technology / Volume 41 / Number 3P2 / May 2002 / Pages 442-444

Biology / Proceedings of the Sixth International Conference on Tritium Science and Technology Tsukuba, Japan November 12-16, 2001

Solid state spin trapping method was applied for measuring the scavenging activity of hydroxyl (OH) radical formed by the beta ray from tritiated water with four tea catechins, (−)-epicatechin (EC), (−)-epicatechin gallate (ECg), (−)-epigallocatechin (EGC) and (−)-epigallocatechin gallate (EGCg). The activity was in the order of EGCg > ECg > EGC > EC. This is consistent with the order of the number of phenolic hydroxyl groups in each catechin molecule. However, it was shown that EGCg had stronger activity than others, which suggested the interaction between the B ring and the gallate group.

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