In the prototype fast breeder reactor Monju, fuel reactivity worth was measured at six positions as the reactivity corresponding to the differences of critical control rod positions between cores with and without a dummy fuel subassembly. In this paper, the measurements are evaluated in detail, and their reliability and usefulness as validation data for fast reactor neutronics design methodologies are examined through a comparison with calculations. Calculated-to-experiment values and their uncertainty of fuel reactivity worth were 0.97 to 1.02 and 4% to 6%. Through this study, the consistency and reliability of the measurements and calculations were confirmed.