The potential use of a pulsed neutron source (PNS) to measure reactivity during nuclear fuel loading as a means to prevent core loading errors has been studied at the GUINEVERE facility. This facility couples the deuteron accelerator GENEPI-3C to the fast neutron subcritical reactor VENUS-F at the Belgian Nuclear Research Center SCK·CEN. The 14-MeV neutrons are produced in the reactor core center via fusion reactions. PNS experiments were performed in five reactor configurations corresponding to the different loading steps of VENUS-F. The evolution of the neutron flux during these PNS experiments was measured by several 235U fission chambers in various positions in the inner and outer reflector and analyzed using the area-ratio method. The results show that, despite strong spatial effects, a strong correlation between the reactivity values given by the area-ratio method and some reference reactivity values remains throughout the reactor unloading. Monte Carlo simulations were first validated by comparison with the data and then used to investigate the sensitivity of the method to a core loading error. First results show that some loading errors could be experimentally detected using a PNS.