Charge exchange spectroscopy has been widely used in fusion devices to measure ion temperature, and toroidal and poloidal flow velocities of plasma. For local measurement, especially in the core region of the plasma, the spectrum emitted by the charge exchange reaction between the main plasma ions or impurity ions and the intentionally injected neutral beam should be analyzed so that parameters can be accurately deduced. Since the line-integrated spectrum signal through the line of sight of the diagnostic optics usually contains an unnecessary overlapped spectrum signal, referred to as the background signal, that typically originates from the plasma boundary region, a beam modulation technique is commonly applied to separate the background signal from the measured spectrum. Recently, it has been demonstrated in the KSTAR tokamak that a two-Gaussian fitting (TGF) method can be applied to analyze the spectrum and deduce plasma ion temperature and toroidal rotation velocity profiles of reasonable accuracy without beam modulation. It has been realized that the measurement result by the TGF method can be alternatively used to investigate plasma transport dynamics when beam modulation is prohibited to avoid any possible disturbance inhibiting robust plasma control and stable operation of the neutral beam injection system.