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Preliminary characterizations on Li isotope separation with Li ionic conductors were carried out. Three types of Li ionic conductors, which were a spinel Li₄Ti₅O₁₂, a perovskite La_0.55Li_0.35TiO₃ and a ramsdellite Li₂Ti₃O₇ from the Li₂O-TiO₂ system, were selected. Electric conductivity and Li isotope separation efficiency were measured for each of the Li ionic conductors. It was shown from the results that each Li ionic conductor has sufficient electric conductivity and high Li isotope separation efficiency. Li isotope separation factors of three of the Li ionic conductors compare with that of the amalgamation process with mercury.