Loss-cone boundaries have been directly measured using a newly developed diagnostic device in the tandem mirror GAMMA 10. Double loss boundaries clearly appeared in the velocity space of the end-loss ions in the experiment without plug ECRH, and upon turning on the ECRH, the double boundaries changed to a single loss boundary. From a microscopic viewpoint of the ion distribution function, it was verified that plug potential was created turning on ECRH without producing sloshing ions by neutral beam injection. Time evolution of plasma potentials is discussed.