Tritium production-rate (TPR) distributions of 6Li, 7Li and NLi were measured in a Be-sandwich Li2O assembly. The Be-region of 50.8 mm-thick was sandwiched by 50.6 and 506 mm-thick Li2O regions. Tritium production rates along the central axis were measured by four techniques, i.e., liquid scintillation method with Li2O pellets, self-irradiation method with LiF TLDs, Li-glass scintillators and a small sphere NE213 detector. The TPRs measured by the four methods agreed well with each other within the experimental errors. The calculation was performed by DOT3.5 with the nuclear data files of JENDL-3T and ENDF/B-IV. An agreement is observed between the calculated and measured TPRs within the experimental errors except near the Be-region.