To determine the accuracy of the neutron transport computer code used to predict 16N production rates, data on 16O(n, p)16N reaction rates were obtained by performing an 16O activation experiment. Measured absolute 16N production rates are compared to the calculated data. The comparison indicates that calculations using ENDF/B-II data consistently overestimate 16N production rates by a factor that ranges from 1.06 to 2.00. Good agreement between measurements and ENDF/B-IV calculations reveals that the latest version of the nuclear data used in the code is remarkably improved over previous versions.