Measurements of 243Am and 235U fission reaction rates are conducted with the use of two single fission chambers in the solid-moderated and solid-reflected core at the Kyoto University Critical Assembly (KUCA). Critical irradiation experiments of 243Am and 235U foils are carried out, and the measured result of 243Am/235U is 0.0424 ± 0.0019; also, calculation/experiment values between calculated (MCNP6.1 with JENDL-4.0, ENDF/B-VIII.0, and JEFF-3.3) and measured results of 243Am/235U range among 0.93 ± 0.04, 0.94 ± 0.04, and 0.93 ± 0.04, respectively. Through a comparison between the measured and calculated results, the 243Am fission cross-section data of the three major nuclear data libraries are successfully validated, demonstrating the same accuracy as that of previous minor actinide irradiation experiments at KUCA. Importantly, the comparison also provides the complemental data of integral experiments of 243Am fission reaction rates that confirm the accuracy of the 243Am fission cross-section data.