A new conceptual design of a tritium permeation test module assembly was developed, for simulation of tritium permeation in the real plasma facing components and validation of the models and codes for evaluation of the tritium permeation. The assembly was designed for tests using powerful ion sources, which has a capability to simulate condition relevant to that of the ITER divertor. The heat load test of the prototype module has been performed using an electron beam to verify thermal and mechanical behavior of the bonded structure and to assess its structural integrity under the cyclic heat loads. Then, the first tests using tritium ion beam generated by the TPE apparatus at TSTA/LANL with the prototype module was performed and procedure to measure tritium permeated was established. Considerations for tests using the target module with defects generated by neutron irradiation or accelerated ion beam irradiation will be also taken in the new module design.