On August 24, 1993, the gas chromatography based Isotopic Separation System (ISS) built in Canada by CFFTP was delivered to the Forschungszentrum in Karlsruhe (FZK) to form part of the Tritium Laboratory Karlsruhe (TLK) infrastructure. The system was successfully reassembled at Karlsruhe, and has been commissioned with a mixture of hydrogen and deuterium. To date, four experimental campaigns have been carried out which show that at liquid nitrogen temperatures, the system is capable of separating 120 I batches of H2, HD and D2. During 1995, the ISS will complete licensing and will be prepared for tritium service.