The inherent delays in components of fuel processing subsystems of fusion reactor on the pulse operation were simulated to analyze the time dependent tritium inventory in the tritium storage with the time in the throughput capacity of the fuel processing to that required to satisfy pulse operation cycle repetition. The simulation results of inherent delays in components of fuel processing subsystems demonstrates that tritium inventory in the tritium storage is influenced by a cumulative time lag system responses on the pulse operation continuity even when adoption of duty cycle is sustained. To satisfy pulse operation up to long pulse specified the capacity of the fuel processing subsystems and tritium storage will need to be increased by a number of fuel storage beds. Variation with the time of tritium product from isotope separation subsystem determines the time dependent characteristic of tritium inventory in the tritium storage. Representative value defined by the inherent system responses provides an information of optimum condition of pulse operation campaign.